YOGYAKARTA Network culture is one of the techniques used in the agricultural world to increase the type of plant species. This technique was born from the research results of botanical scientists who ultimately helped produce certain plants that were superior. The advantages in question can vary, ranging from producing plants that are resistant to disease, producing many plants in a short time, and much more. For people who want to try it, first know the stages of tissue culture.

In the book Technical Guidelines for Expanding Forest Plants through Network Culture published by the Ministry of Environment and Forestry, it is explained that tissue culture is a technology used in biotechnology activities.

Network culture has several benefits in the botanical world, for example, accelerating the process of rapidly breeding/regeneration of plants and much without depending on the season. How to multiply these plants use the vegetative part of plants.

Dalam website resmi Dinas Agricultural Kabupaten Buleleng dijelaskan bahwa tahap kultur jaringan tanaman dibagi menjadi beberapa yakni sebagai berikut.

Media is a factor that determines success. At this stage, a growing medium must be provided consisting of a mixture of various substances, namely a mixture of mineral salt containing macro elements and microamino acids, vitamins, sugar, and plant hormones.

is an explicit collection from the part of the plant that has been taken to be cultured. The explicit part taken is the shoots.

Sterilization is needed so that there is no contamination. All activities in tissue culture must be carried out in a sterile place, namely at the laminar flow. Not only places, the tools used are also mandatory to be sterile. Sterilization of equipment can take advantage of ethanol that is sprayed to the surface of equipment evenly. In addition, officers or technicians who will hold a network culture must also be sterile.

Multiplication is the procurement of shoots. This activity is carried out to increase the number of shoots by planting an explicit in the media. It can also be done by doing certain stimuli so that new shoots appear.

This activity needs to be carried out in a sterile place, namely at the laminar flow so that there is no contamination that triggers growth failure.

This stage is the transfer of the explicit out of the aseptic room to partner. How to do it must be careful and gradual.

In Indonesia, tissue culture has been carried out for a long time to develop agricultural or plantation products. Some plants that have been developed through the culture of coffee, cocoa, tea, and forestry plants such as tea, sandalwood, agarau, etc.

Although able to produce superior seeds, this technique has several weaknesses. For example, the costs required are quite large. In addition, not everyone can do this technique. In addition, the results of the plants that have been successfully developed have weak roots.

That's information related to the stages of network culture. Visit VOI.id to get other interesting information.


The English, Chinese, Japanese, Arabic, and French versions are automatically generated by the AI. So there may still be inaccuracies in translating, please always see Indonesian as our main language. (system supported by DigitalSiber.id)

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